Figure 6
Knocking down BMPER with siRNA reduced migration and invasion capacities and matrix production of fibroblasts.
(a) Cell invasion capacity of MRC5 cells transfected with BMPER siRNA or control siRNA (*P < 0.05, data are mean and SEM, unpaired t test, n = 3 times). (b) Representative images of Invasion (Scale bars, 200 μm). (c) Cell migration capacity of MRC5 cells transfected with BMPER siRNA or control siRNA was assayed with a Boyden chamber (*P < 0.05; data are mean and SEM, unpaired t test, n = 3 times). (d) Representative images of migration (Scale bars, 200 μm). (e) HA concentration of MRC5 cells transfected with BMPER siRNA or control siRNA (HA, Hyaluronan; **P < 0.01; data are mean and SEM, unpaired t test; n = 3 times). (f) HA concentration of normal and IPF lung fibroblasts transfected with BMPER siRNA or control siRNA (HA, Hyaluronan; *P < 0.05; **P < 0.01; data are mean and SEM, unpaired t test, normal n = 5 subjects and IPF n = 5 patients).
