Figure 5
Model of the molecular mechanism for the wing mutagenesis induced by HHP treatment in D. melanogaster.
HHP stress induces 285 differentially expressed genes in the mutants. Among them, 6 genes, including sca, hth, osa, dad, ilk, and stck have a direct relationship with wing development. In addition, Numb, tom, sca, bun and nedd4 genes have an indirectly role in D. melanogaster wing development through Notch signaling pathways. The sca gene is common to both. The Notch signaling pathway also affects D. melanogaster wing phenotype via the vg target gene and HHP stress induces two point mutations in the vg gene. The two mutations are synonymous mutations. Furthermore, the four bases deletion in the exon 1 of vg gene resulting in the change of reading frame and the consequent failure to generate functional protein. An insertion sequence between the second and third exon of vg indirectly affect the function of protein. The coordinated interaction of these abnormal genes is crucial for the wing mutagenesis induced by HHP treatment in D. melanogaster.
