Figure 4

After transplantation of EPCs and NPCs derived from human ASCs, the engraftment of differentiated cells was identified with double staining (arrowheads) of human chromatin (hChromatin, green) and by specific antibodies against RECA (n = 5), NeuN (n = 5) and GFAP (n = 5) (red).
(a) The E+N combined therapy promoted endogenous neurogenesis as indicated by increased nestin staining specific for rat neural stem cells, but not human nestin. (b) The combination of E+N also increased angiogenesis as demonstrated by increased isolectin IB4 staining (n = 5). (c) Scale bar: 50 μm.