Figure 6 | Scientific Reports

Figure 6

From: Synergy of endothelial and neural progenitor cells from adipose-derived stem cells to preserve neurovascular structures in rat hypoxic-ischemic brain injury

Figure 6

The combination of EPCs and NPCs increased transmigration in a Boyden chamber under normoxic conditions and the application of the hypoxia mimetic reagent DFO further promoted the mobility in the E+N group (n = 5).

(a) Different types of cells with or without DFO treatment were harvested to assess the potential gene expression in angiogenesis and neurogenesis signaling pathways for ANG1, ANG2, NRP1, CXCR4 and FGFR1 (n = 3). (b) Specific inhibitions of angiogenic (NRP1 inhibiting peptide, DG2) and neurogenic (AMD3100, a CXCR4 inhibitor and SU5402, an FGFR1 inhibitor) signaling were applied to EPCs or NPCs (n = 5). (c) Synergistic interactions were originated from EPCs via NRP1 signaling and from NPCs by CXCR4 and FGFR1 signaling pathways. Scale bar: 200 μm. *p < 0.05 compared to undifferentiated ASCs under normoxia. #p < 0.05 compared to undifferentiated ASCs under hypoxia.

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