Figure 4

Chemical bonding and molecular structures identified by FT-IR and Raman spectroscopy.
(a) FT-IR traces of CNP-PEI, CNP-PAMAM−, CNP-Bis-MPA, CNP-PEG, respectively (top to down). (b) Overlaid Raman spectra of PEI and CNP-PEI, PAMAM− and CNP-PAMAM−, Bis-MPA and CNP-Bis-MPA, PEG and CNP-PEG and CNP-bare, respectively (top to down). (c–f) Stability of CNPs in acidic, neutral and basic pH and in presence of fetal bovine serum (FBS). Particle stability was followed by hydrodynamic diameter determination at various time points including 0, 24, 48 and 72 h. Dav = hydrodynamic diameter.