Figure 3
From: Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
GSL expression in iPSCs, their parental cells and iPSC-NSCs.
(A) Immunocytochemical analyses of NANOG, Globo H, H type1 antigen and SSEA-1 in MRC-5, MRC-iPSCs, MRC-iPSC-EB outgrowth and MRC-iPSC-NSCs. Inserted images are nuclear staining by DAPI. Scale bar is 100 μm. (B) Flow cytometric analyses of GSLs in MRC-5, MRC-iPSCs and MRC-iPSC-NSCs. SSEA-4, Globo H, H type1 antigen and SSEA-1 were analyzed using GSL-specific antibodies versus the isotype controls. Cells stained with specific antibodies are shown in red and isotype antibodies are shown in black. (C) Time course analyses by flow cytometry of SSEA-4, Globo H and H type1 antigen during the process of differentiation from iPSCs to NSCs. Black columns represent MRC-iPSCs and white columns, UtE-iPSCs. N.D. is “not detected”.
