Figure 5 | Scientific Reports

Figure 5

From: Identification of cellular microRNA-136 as a dual regulator of RIG-I-mediated innate immunity that antagonizes H5N1 IAV replication in A549 cells

Figure 5

miR-136 activates innate immunity independent of TLRs pathway.

(A) A549 cells were cotransfected with naked miR-136 and either NC or 2-O-methyl modified miR-136. At 24 hours following cotransfection, IL-6 and IFN-β mRNAs were detected. (B) Measurement of IL-6 and IFN-β mRNA expression levels in 293T cells exposed to miR-136. (C,D) Chloroquine (10 μM)-treated A549 cells were transfected with 60 nM miR-29a or miR-136 and IL-6 and IFN-β mRNA expression levels were determined by RT-qPCR. Data are presented as mean ± SD from triplicate independent experiments. **P < 0.01, ***P < 0.001, as determined by one-way ANOVA with Bonferroni’s multiple comparison test (A,C,D) or Student’s t test (B). 2′M, 2′-O-methyl modified; chl, chloroquine; ns, no significant.

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