Figure 8

Neutralization of endogenous miR-136 negatively regulates RIG-I-dependent signalling.

(A) 12-well planted A549 cells were subjected to miR-136 inhibitors or inhibitor negative control transfection using a concentration of 60 nM. 24 hours after transfection, cellular RIG-I, IFN-β and IL-6 mRNA levels were evaluated by RT-qPCR. (B,C) 12-well planted A549 cells were treated by the same procedure as above. 24 hours after transfection, cells were infected with H5N1/HM virus at an MOI of 0.5. At 36 hours postinfection, cells were collected and proceed with RNA extraction. NP mRNA abundance and vRNA numbers were evaluated by RT-qPCR assays. (D) 12-well planted A549 cells followed the same procedure as in (A). VSV was inoculated using an MOI of 0.01 and plaque assays were performed 24 hours later. The results are presented as mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01, as determined by performing a Student’s t test.