Figure 1 | Scientific Reports

Figure 1

From: Conformational switch of polyglutamine-expanded huntingtin into benign aggregates leads to neuroprotective effect

Figure 1

FKBP12 decreases the amyloidogenicity of Htt(Q)43 and promotes the formation of amorphous aggregates.

(a) A schematic representation of the GST-tagged constructs expressing Huntingtin exon 1 with 25 or 43 polyglutamine tracts. (b) The fold change in ThT fluorescence between Htt(Q)43 and Htt(Q)43/FKBP12 after GST removal, on days 1 and 7. All data are presented as means with SEM (n = 3). *p < 0.05, Student’s t-test (c) TEM images of the Htt(Q)25 and Htt(Q)43 proteins in the presence/absence of FKBP12 after GST removal. Morphological changes of the Htt(Q)43 proteins are depicted as follows: red arrow, protofibrils; black arrow, fibril; blue arrow, amorphous aggregates. Scale bars represent 100 nm.

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