Table 1 The primary antibodies used for western blot analysis and immunohistochemistry.
Antibody | Specificity | Type | Dilution | Source |
|---|---|---|---|---|
p-TrkB | Phosphorylated TrkB at Tyr706 | pAb | 1:1000 | Santa Cruz |
TrkB | Total TrkB | pAb | 1:1000 | Biovision |
p-Erk1/2 | Phosphorylated Erk1/2 | pAb | 1:1000 | Cell Signaling |
Erk1/2 | Total Erk1/2 | pAb | 1:1000 | Cell Signaling |
p-Akt(473) | Phosphorylated Akt at Ser473 | pAb | 1:1000 | Cell Signaling |
Akt | Total Akt | pAb | 1:1000 | Cell Signaling |
p-PKC | Phosphorylated PKC | pAb | 1:1000 | Cell Signaling |
PKC | Total PKC | pAb | 1:1000 | Cell Signaling |
p-CREB | Phosphorylated CREB at Ser133 | pAb | 1:1000 | Cell Signaling |
CREB | Total CREB | pAb | 1:1000 | Cell Signaling |
Arc | Total Arc | pAb | 1:1000 | Calbiochem |
pS396 | Phosphorylated tau at Ser396 | pAb | 1:1000 | Biosource |
pS404 | Phosphorylated tau at Ser404 | pAb | 1:1000 | Biosource |
pS262 | Phosphorylated tau at Ser262 | pAb | 1:1000 | Biosource |
pT231 | Phosphorylated tau at Thr231 | pAb | 1:1000 | Biosource |
pT205 | Phosphorylated tau at Thr205 | pAb | 1:1000 | Biosource |
tau1 | Nonphosphorylated tau | mAb | 1:1000 | Millipore |
PP2A | Total PP2A | mAb | 1:1000 | Cell Signaling |
p-PP2A(Y307) | Phosphorylated PP2A at Y307 | mAb | 1:1000 | Abcam |
t-APP | Total APP | mAb | 1:1000 | Millipore |
p-APP | Phosphorylated APP at Thr668 | pAb | 1:1000 | Cell Signaling |
α/β-CTF | Anti-α/β-CTF | pAb | 1:1000 | Iqbal |
PS1 | Presenilin-1 | pAb | 1:1000 | Cell Signaling |
BACE1 | BACE1 | pAb | 1:1000 | Cell Signaling |
DM1A | α-tubullin | mAb | 1:2000 | Sigma |
4G8 | Anti-Aβ | mAb | 1:200 | Iqbal |
GSK-3 | Total GSK-3α/b | pAb | 1:1000 | Cell Signaling |
p-GSK3β(S9) | Phosphorylated GSK3β at Ser9 | pAb | 1:1000 | Cell Signaling |
p-GSK3β(Y216) | Phosphorylated GSK3β at Tyr216 | mAb | 1:1000 | Upstate |
P35/25 | Total P35/25 | pAb | 1:1000 | Cell Signaling |
CDK5 | Total CDK5 | mAb | 1:1000 | Abcam |
PSD93 | Total postsynaptic density 93 | pAb | 1:1000 | Abcam |
PSD95 | Total postsynaptic density 95 | pAb | 1:1000 | Abcam |
GluR1 | Total GluR1 | mAb | 1:1000 | Millipore |
GluR2 | Total GluR2 | mAb | 1:1000 | Millipore |
NR1 | Total NMDA receptor 1 | pAb | 1:1000 | Abcam |
NR2A | Total NMDA receptor 2A | pAb | 1:1000 | Abcam |
NR2B | Total NMDA receptor 2B | pAb | 1:1000 | Abcam |
Synapsin I | Total Synapsin I | pAb | 1:500 | Millipore |
Synaptophysin | Total Synaptophysin | mAb | 1:1000 | Sigma |
M receptor | Total M1 receptor | pAb | 1:100 | Sigma |
