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Figure 1

From: Activation of Vago by interferon regulatory factor (IRF) suggests an interferon system-like antiviral mechanism in shrimp

Figure 1

Expression of IRF in L. vannamei tissues.

Tissue distribution of IRF was detected by RT-PCR (A), western-blot (B) and real-time PCR (C). Real-time RT-PCR was performed in triplicate for each sample using EF-1α gene as internal control by the Livak (2−△△CT) method. Expression levels were provided as the mean fold changes (means ± SD, n = 3) relative to that in hemocytes, which was set as 1.0. (D,E) Expression profiles of IRF after WSSV and poly (I:C) challenges analyzed by real-time PCR. The expression level at 0 h post PBS injection was set as baseline (1.0). **p < 0.01.

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