Figure 2 | Scientific Reports

Figure 2

From: Activation of Vago by interferon regulatory factor (IRF) suggests an interferon system-like antiviral mechanism in shrimp

Figure 2

Dimmerization and subcellular localization of L. vannamei IRF protein.

(A) IRF dimmers in intestines, hepatopancreas and hemocytes (as negative control) were analyzed using native PAGE electrophoresis coupled with western-blot.(B) Interaction between GFP- and V5-tagged IRF proteins analyzed by co-immunoprecipitation (Co-IP). (C) The nuclear translocation of IRF after WSSV or poly (I:C) stimulation. Drosophila S2 cells expressing GFP-tagged IRF (green) were treated with Hochest 33258 to counterstain nuclei (blue) and observed under confocal laser scanning microscope. (D) Dimmerization of IRF in shrimp hepatopancreas analyzed by native PAGE electrophoresis and western-blot.

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