Figure 3
Transcriptional regulatory function and antiviral acitivity of L. vannamei IRF.
(A) Activation of ISRE-containing promoter by IRF detected using dual luciferase reporter assays performed on Drosophila S2 cells. (B) Transcriptional regulatory effects of L. vannamei IRF on mammalian type I and III IFNs in HEK293T cells. (C) Activation of Mx1 protein in L. vannamei IRF-expressed HEK293T cells detected by western-blot. (D) L. vannamei IRF triggers an antiviral state in mammalian cells. HEK293T cells were transfected with pcDNA-IRF and pcDNA 3.1 (as control) and infected with Tiger frog virus (TFV). Left panel, pathological signs of TFV-infected cells at 24, 48 and 72 h post infection; right panel, titers of the released TFV in the cell supernatants analyzed using TCID50 method.
