Figure 2
From: An improved method for detecting circulating microRNAs with S-Poly(T) Plus real-time PCR
Comparison of sensitivity in miRNA assay between S-Poly(T) and S-Poly(T) Plus methods.
(A) The expression levels of miR-103a-3p, miR-27b-3p, miR-126-3p, miR-34b-5p, miR-223-3p, miR-150-5p and SNORD44 were determined using total RNAs isolated from human HEK293A cells. (B) The expression levels of five human miRNAs of miR-103a-3p, miR-27b-3p, miR-126-3p, miR-223-3p, miR-150-5p and a spiked-in Caenorhabditis elegans miRNA cel-miR-54-5p were measured using total RNAs prepared from 24 healthy human serum mixture.
