Figure 5

Optimization of serum RNA extraction using the co-precipitant, glycogen.

Various concentrations of glycogen were applied to enhance the precipitation of serum RNA, which were used for detection of miRNAs using S-Poly(T) (A) and S-Poly(T) Plus (B). S: serum, Gly: glycogen and -RT: without reverse transcriptase. The concentrations of glycogen were 240 ~ 1.875 μg/ml. (C) Serum was subjected to the first or second extraction (see Method section for definitions for first/second extraction). The isolated RNA was assayed for miRNA levels using S-Poly(T) Plus method.