Figure 1 | Scientific Reports

Figure 1

From: A reactive oxygen species activation mechanism contributes to JS-K-induced apoptosis in human bladder cancer cells

Figure 1

JS-K inhibits cell proliferation and promotes cell apoptosis.

(A) JS-K-induced apoptosis in T24 and UM-UC-3 cells at 24 h as visualized by microscopy (100×). (B) Cell proliferation, as detected by the CCK-8 assay, was suppressed in a concentration- and time-dependent manner after JS-K treatment. (C) Cytotoxicity of JS-K was determined by an LDH assay. The data indicate that JS-K affected bladder cancer cells in a concentration-dependent manner. (D) JS-K-induced cell apoptosis was analyzed by flow cytometry. After 24 h treatment with JS-K, apoptosis of T24, UM-UC-3 and SV-HUC-1 cells were measured and the data indicate that JS-K induced apoptosis of T24 and UM-UC-3 but had no significant effect on SV-HUC-1 cells. (E) JS-K did not inhibit cell proliferation in SV-HUC-1 human nephric tubule cells. The data are presented as the mean ± SD for at least three independent experiments. Double asterisks (**) indicate an extremely significant difference (P < 0.01).

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