Figure 6

JS-K enhanced the chemo-sensitivity of doxorubicin in bladder cancer cells.

(A) Colony formation assay with different treatments of JS-K and doxorubicin in bladder cancer cells. Representative graphs (left). The clones were quantified and the results are presented in a graph (right). (B) Cell viability was measured by a CCK-8 assay after treatment with JS-K and doxorubicin. (C) Changes in the IC50 of doxorubicin. (D) Cytotoxicity of different drug combinations. (E) Caspase-3/7 activity of bladder cancer cells with various drug concentrations and treatment durations. (F) Apoptosis of T24 and UM-UC-3 cells induced by the drugs individually and in combination at different concentrations and treatment durations. The data are presented as the mean ± SD for at least three independent experiments. Single asterisks (*) indicate a significant difference (P < 0.05) and double asterisks (**) indicate an extremely significant difference (P < 0.01).