Figure 2
From: Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels
Images of aligned cell sheets and resulting TEBV.
(A) SEM image of nanopatterned PDMS; (B,C) Phase images of aligned hMSCs grown on nanopatterned PDMS, (D) hMSC sheets wrapped around 1.3 mm diameter glass rod; (E,F) SEM images of circumferentially aligned hMSC in outer layer of TEBV; (G,H) Confocal images showing phalloidin staining (Red) and DAPI (Blue) of TEBV; (I–L) SEM images of various TEBV sections demonstrating that the cell sheets were well fused to create multiple layers of the vessel wall with confluent cell density throughout the vessel from the lumen to the exterior ((I): TEBV; (J): longitudinal section showing lumen; (K): longitudinal section showing the outer wall; and (L): cross-section)
