Figure 5

Differentiation of myeloid NK precursors from hematopoietic stem/progenitor cells in vitro.

CD34⁺ cells were purified from human CB and cultured in NK differentiation medium. Cells in the culture were analyzed by flow cytometry for CD56 and CD36 everyday initially and every two to four days later. Shown are CD56 versus CD36 staining profiles at the indicated time points (A) and percentages of CD36⁺, CD56^lowCD36⁺ and CD56^highCD36⁻ cells over time (B). (C) Cells in the culture were analyzed for CD56, CD36, CD33, NKG2D, NKp46, CD14 and HLA-DR every 7 days. Shown are staining profiles of CD56 versus CD36, CD33, NKG2D, NKp46, CD14 and HLA-DR at day 14. The experiments were repeated at least 3 times with similar results.