Figure 3
From: Trapping toxins within lipid droplets is a resistance mechanism in fungi
LD-deficient yeast mutants are hypersusceptible to lipophilic toxins.
(A) The LD morphology of C. albicans mutant strains stained with Nile red as revealed by CLSM observation. (B) Measurements of LD size in C. albicans mutant strains, which were determined by Nile red staining and flow cytometry. Black line, negative control; red line, BWP17; green line, dga2Δ/dga2Δ; blue line, are2Δ/are2Δ; and purple line, lro1Δ/lro1Δ. (C) The susceptibility of C. albicans LD-deficient mutant strain dga2Δ/dga2Δ and its parent strain BWP17 to HA-mediated photodynamic inactivation. (D) The susceptibility of S. cerevisiae mutant strains to HA-mediated phototoxicity. Data represent mean values ± SD from three independent replicates. Student’s t test was used to determine significant differences. *p < 0.05, ***p < 0.001.
