Figure 3
From: Distinct microbiological signatures associated with triple negative breast cancer
Validation of PathoChip hybridization results by PCR.
Primers for PCR amplification were designed from the conserved and specific probes that hybridized to the targets used in the PathoChip screen. The heat map across the cancer and control samples for the probes from which the PCR primers were designed are shown in the left panel for each of the PCR amplification gel picture. Amplified PCR product validated the PathoChip hybridization results. MC: matched control (adjacent non-cancerous breast tissue from breast cancer patients); NC: non-matched control (breast tissue from healthy individuals). Non-template control (NTC) using sterile water was used to rule out any contamination in the PCR reaction.
