Figure 3 | Scientific Reports

Figure 3

From: Fructose 1,6-bisphosphate, a high-energy intermediate of glycolysis, attenuates experimental arthritis by activating anti-inflammatory adenosinergic pathway

Figure 3

Inhibition of A2aR abrogates anti-inflammatory effects of FBP.

C57BL/6 mice were pre-treated with A2a receptor antagonist (8,3-CSC, 1 mg.kg−1) 1 h before administration of FBP (100 mg.kg−1), which was given 24 h and 30 min before arthritis induction. (A,B) Neutrophils infiltration into the joint analysed 6 h after arthritis induction. (C,D) Mechanical hyperalgesia analysed 6 h after arthritis induction. (E) Articular oedema determined at different times after arthritis induction. (F–H) Intra-articular TNF-α (F), IL-6 (G) and IL-10 (H) tissue levels determined 6 h after arthritis induction. Data represent mean ± s.e.m., n = 5 mice per group. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with vehicle group; #P < 0.05 and ###P < 0.001 compared with FBP group.

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