Figure 5
Inhibition of ectonucleotidases abrogates anti-arthritic effect of FBP.
C57BL/6 mice were pretreated with CD39 (ARL67156, 2 mg.kg−1) or CD73 (adenosine 5′-(α,β-methylene) diphosphate 4 mg.kg−1) inhibitors 1 h before FBP treatment protocol used prior to arthritis induction by zymosan injection (30 μg/knee joint). (A,B) Representative histograms showing CD39 (A) and CD73 (B) expression on blood CD11b+ leukocytes from mice treated or not with FBP (100 mg.kg−1) 6 h after arthritis induction. (C,D) Neutrophils infiltration into the joint analysed at 6 h after arthritis induction. (E,F) Mechanical hyperalgesia analysed 6 h after arthritis induction. (G,H) Articular oedema determined at different times after arthritis induction. (I–K) Intra-articular TNF-α (I), IL-6 (J) and IL-10 (K) tissue levels determined 6 h after arthritis induction. Data represent mean ± s.e.m., n = 5 mice per group. P < 0.05, **P < 0.01 and ***P < 0.001 compared with vehicle group; #P < 0.05, ##P < 0.05 and ###P < 0.001 compared with FBP group.
