Figure 3
Expression of AR in quadriceps muscle and spinal cords of SBMA mouse model.
RT-qPCRs were performed on total RNA extracted from quadricep muscles (A) or whole spinal cords (B) of male non-transgenic (Wt) mice and of AR113Q mice at 9 (corresponding to presymptomatic stage) or 24 (corresponding to symptomatic stage) weeks of age. As additional control, quadriceps muscles of female AR113Q mice at 24 weeks were used (n = 5). Data have been normalized to the amount of GAPDH mRNA, expressed relative to the levels determined in Wt mice at 9 weeks taken as internal reference and expressed as fold changes. Data are means ± SD of three independent replicates for Wt and AR113Q mice at 9 weeks and of five independent replicates for Wt and AR113Q mice at 24 weeks. (A) RT-qPCR on AR mRNA expression levels in quadricep muscles. (B) RT-qPCR on AR mRNA expression levels in spinal cords.
