Figure 5
Expression of pro-autophagic chaperones in quadriceps muscle of SBMA mouse model.
RT-qPCRs were performed on total RNA extracted from quadricep muscles of male non-transgenic (Wt) mice and of AR113Q mice at 9 (corresponding to presymptomatic stage) or 24 (corresponding to symptomatic stage) weeks of age. As additional control, quadriceps of female AR113Q mice at 24 weeks were used (n = 5). All animals were age-matched. Data have been normalized to the amount of GAPDH mRNA, expressed relative to the levels determined in Wt mice at 9 weeks taken as internal reference and expressed as fold changes. Data are means ± SD of three independent replicates for Wt and AR113Q mice at 9 weeks and of five independent replicates for Wt and AR113Q mice at 24 weeks. (A) RT-qPCR on HSPB8 mRNA expression levels in quadricep muscles. *p < 0.05 vs. 24 weeks Wt mice; °°p < 0.001 vs. 9 weeks AR113Q mice. (B) RT-qPCR on HSPB2 mRNA expression levels in quadricep muscles. **p < 0.001 vs. 24 weeks Wt mice; °°p < 0.001 vs. 9 weeks AR113Q mice. (C) RT-qPCR on HSPB3 mRNA expression levels in quadricep muscles. **p < 0.001 vs. 24 weeks Wt mice; °°p < 0.001 vs. 9 weeks AR113Q mice.
