Figure 6
Expression of BAG1 and BAG3 co-chaperones in quadriceps muscle of SBMA mouse model.
RT-qPCRs were performed on total RNA extracted from quadricep muscles of male non-transgenic (Wt) mice and of AR113Q mice at 9 (corresponding to presymptomatic stage) or 24 (corresponding to symptomatic stage) weeks of age. As additional control, quadriceps of female AR113Q mice at 24 weeks were used (n = 5). All animals were age-matched. Data have been normalized to the amount of GAPDH mRNA, expressed relative to the levels determined in Wt mice at 9 weeks taken as internal reference and expressed as fold changes. Data are means ± SD of three independent replicates for Wt and AR113Q mice at 9 weeks and of five independent replicates for Wt and AR113Q mice at 24 weeks. (A) RT-qPCR on BAG1 mRNA expression levels in quadricep muscles. **p < 0.001 vs. 24 weeks Wt mice; °p < 0.05 vs. 9 weeks AR113Q mice. (B) RT-qPCR on BAG3 mRNA expression levels in quadricep muscles. **p < 0.001 vs. 24 weeks Wt mice; °°p < 0.001 vs. 9 weeks AR113Q mice. (C) BAG3:BAG1 relative ratio of mRNA expression levels in quadricep muscles. Data represent variations of the relative levels of BAG3 and BAG1 normalized over the relative BAG3 and BAG1 levels of age-matched 9 weeks Wt mice. **p < 0.001 vs. 24 weeks Wt mice; °°p < 0.001 vs. 9 weeks AR113Q mice.
