Figure 2 | Scientific Reports

Figure 2

From: An in vitro assessment of liposomal topotecan simulating metronomic chemotherapy in combination with radiation in tumor-endothelial spheroids

Figure 2

Endothelial cells are more sensitive to low concentrations of TPT alone and in combination with radiation in comparison to tumor cells.

Effect of TPT treatment on the viability of tumor (A) and endothelial cells (B) was assessed with or without radiation (3 Gy) exposure in a 72h MTT assay. IC50 values obtained from the MTT assay indicate that X-ray irradiation post TPT treatment considerably decreases the viability of both tumor and endothelial cells. Long term survival studies further validate the impact of radiation (3y) on tumor and endothelial cell colony formation with and without TPT at ½ or ¼ of the IC50 concentrations. For the survival assay, cells were exposed to drug and/or radiation and then grown in drug-free medium for 10–14 days. Colonies (>50 cells) were stained with crystal violet and counted (Materials and Methods). Data represent means ± SD (n = 3). Significant changes were recorded as **p < 0.01; *p < 0.05.

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