Figure 3 | Scientific Reports

Figure 3

From: An in vitro assessment of liposomal topotecan simulating metronomic chemotherapy in combination with radiation in tumor-endothelial spheroids

Figure 3

Tissue-like integrated morphology and development of extracellular matrix prevents penetration of TPT in the TES.

(A) Tumor cell only and tumor-endothelial cell (TES) spheroids were incubated in 2.5 μM TPT for 6 hours and extent of uptake was assessed using fluorescent confocal imaging. Efficient uptake of TPT imaged at excitation wavelength 410 nm was observed in tumor cell spheroids. There was minimal penetration of the fluorescent TPT (green). Scanning electron micrographs (B), histology (C) and immunostaining for ECM proteins (D) like collagen and fibronectin indicate the compactness as well as the presence of ECM in the TES.

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