Figure 8
HSM restores SOD expression in the lungs of BLM-treated mice and lung fibroblasts.
(a) SOD mRNA expression in the lung tissues was measured on day 21 by real-time RT-PCR. Data are presented as fold changes in gene expression normalized to β-actin. (b) SOD protein expression in the lung tissues was measured on day 21 by Western blotting. Quantitative results are from three independent experiments. (c) Western blotting of SOD protein in MRC-5 cells pretreated with 1% or 2% of HSM extract, prior to treatment with TGF-β1 (5 ng/ml) for 24 h. Relative protein levels were quantified by densitometry and normalized to β-actin. #P < 0.05 versus PBS + EtOH group or untreated (ethanol-treated) cells. *P < 0.05 versus BLM + EtOH group or control cells (TGF-β1–treated).
