Table 1 Primer sequences used for quantitative real-time RT-PCR.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
ASC | GAGCTGCTGACAGTGCAAC | GCCACAGCTCCAGACTCTTC |
Caspase-1 | ACCCTCAAGTTTTGCCCTTTAGAA | TCTGAGGTCAACTTGGACTCCAAC |
Collagen 3α1 | GTTCTAGAGGATGGCTGTACTAAACACA | TTGCCTTGCGTGTTTGATATTC |
IL-1β | GCTCATCTGGGATCCTCTCC | CCTGCCTGAAGCTCTTGTTG |
IL-6 | CCACTTCACAAGTCGGAGGCTTA | GCAAGTGCATCATCGTTGTTCATAC |
IL-18 | ACAACTTTGGCCGACTTCAC | GGGTTCACTGGCACTTTGAT |
NLRP3 | AGAGCCTACAGTTGGGTGAAATG | CCACGCCTACCAGGAAATCTC |
P2X7R | TCTTCCGACTAGGGGACATCT | ATGGGACCAGCTGTCTAGGTT |
SOD | GCAGGGAACCATCCACTT | TGCCCAGGTCTCCAACAT |
TGF-β1 | AAACGGAAGCGCATCGAA | GGGACTGGCGAGCCTTTAGTT |
TNF-α | GTGGAACTGGCAGAAGAGGC | AGACAGAAGAGCGTGGTGGC |
β-actin | GATTACTGCTCTGGCTCCTAGC | GACTCATCGTACTCCTGCTTGC |
