Figure 6 | Scientific Reports

Figure 6

From: A Designed Angiopoietin-1 Variant, Dimeric CMP-Ang1 Activates Tie2 and Stimulates Angiogenesis and Vascular Stabilization in N-glycan Dependent Manner

Figure 6

Dimeric CMP-Ang1 induces angiogenesis and prevents vascular permeability in vivo.

(A–F) One μg of BSA, CA1-3 and COMP-Ang1 were intradermally injected to normal ears (A–C) and hole-punch injury ears (D–F) every 12 hr for 7 days. Blood vessels in normal ear skin (A) and wound margin of ear skin (D) were visualized by CD31 immunostaining. Blood vessel densities (BVD) (B,E) and numbers of different-sized vessels (C,F) in the normal and wound margin of ear skin were measured. Each graph indicates the mean ± SD of five experiments. Scale bars, 100 μm. *p < 0.05 versus BSA. (G,H) Ears were treated intradermally with PBS, LPS, or LPS with CA1-3 or COMP-Ang1 and then Evans blue dye was injected intravenously to visualize plasma leakage. Gross images of Evans blue dye extravasation in ears (G) and its quantification (H). Each graph indicates the mean ± SD of four experiments. *p < 0.05 versus LPS-treated group.

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