Table 1 Biochemical characteristics of Ang1 variants.

From: A Designed Angiopoietin-1 Variant, Dimeric CMP-Ang1 Activates Tie2 and Stimulates Angiogenesis and Vascular Stabilization in N-glycan Dependent Manner

 

Elution, %

Solubility, %

Stability, %

Aggregation, %

CA1-1

> 90

> 90

> 95

Not detectable

CA1-2

> 90

> 90

ND

Not detectable

CA1-3

> 90

> 90

> 95

Not detectable

Fc-A1

> 90

> 90

ND

Not detectable

GCN-A1

> 85

> 90

ND

Not detectable

COMP-A1

> 90

> 90

> 95

> 10%

Native Ang1

> 65

> 70

> 60

> 50%

  1. Elution is the percentage of protein released from streptactin agarose beads. Solubility is the percentage of soluble protein at pH 4.0 in acetic acid buffer. Insoluble proteins were precipitated. Elution and solubility were measured with freshly prepared proteins. Stability is the phosphorylation activity of proteins on Tie2 in HUVECs after being stored at −70° C for 3 months. Aggregation is the percentage of protein aggregates under non-reducing condition in the stacking gel of SDS-PAGE after being stored at −70° C for a month. Three repeats of each experiment showed similar findings. ‘ND’ is ‘not determined’.