Figure 2
From: Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure
Nucleoid components and cholesterol co-sediment in the absence of mitochondrial DNA.
Total mitochondrial lauryl-maltoside lysates from mtDNA containing control cells (a) and ρ° cells (b) were separated on top-down iodixanol gradients. Though some of the nucleoid component are lost or have moved to the top of the gradient in the absence of mtDNA, some still co-sediment at the bottom of the gradient. Pellet preparations from digitonin treated purified mitochondria from ρ° cells were separated on a bottom-up flotation iodixanol gradient (c). The nucleoid-protein and cholesterol fractions remain intact and co-fractionate also in the absence of mtDNA. mtDNA, mitochondrial DNA; TFAM, mitochondrial transcription factor A; SSB, single-stranded binding protein; polG, polymerase Gamma; POLRMT, mitochondrial RNA polymerase; HSP60, heat shock protein 60; coxII, cytochrome oxidase subunit II, PHB, prohibitin; Cyp-D, Cyclophilin D. Protein, DNA and lipid profiles of representative gradients.
