Figure 6
From: Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure
The cholesterol-rich fraction and the associated nucleoid remain intact after the knock-down of mitofilin.
Pellet preparations from digitonin treated purified mitochondria derived from mitofilin siRNA treated HEK293 cells were separated on a bottom-up flotation iodixanol gradient (a). The majority of the cholesterol and corresponding nucleoid components migrate to the same fraction as in control preparations (Fig. 1). Mitofilin knock-down (b). TFAM, mitochondrial transcription factor A; SSB, single-stranded binding protein; coxII, cytochrome oxidase subunit II. Protein, DNA and lipid profiles of a representative gradient.
