Figure 3

A 10 minute exposure to 100% N2 reduces D. melanogaster flight but not climbing, which is rescued by 1% O2.
Flies were exposed to 100% N2 or 99% N2/1% O2 for 10 minutes and recovered for one hour prior to being assayed for climbing or flight. (a) Climbing ability is unaffected by the N2 exposure; however, (b) flight is reduced. (c) Climbing remains unaffected by 99% N2 exposure, while (d) the flight deficit is rescued by the addition of 1% O2. Columns represent the mean ± SEM of the percentage of flies able to climb or fly. Statistical analysis was performed by Student’s t-tests. a = P < 0.001.