Figure 6
Exposure to 99% CO2 reduces D. melanogaster climbing ability for an extended period of time in a dose-dependent manner.
Flies that were exposed to 99% CO2/1% O2 in the exposure apparatus had reduced climbing abilities at various recovery time points. Flies exposed for 10 minutes had reduced climbing through 8 hours, while the climbing of flies that were exposed for 30 minutes was reduced through 16 hours. The 30 minute exposure inhibited D. melanogaster climbing more than the 10 minute exposure. The Air-exposed points represent the average of all controls performed for that time point; however, the statistical analysis (Student’s t-test) was performed with the individual control flies for each exposure length. The data points represent the mean ± SEM of the percentage of flies able to climb. a = P < 0.01 vs. Air control flies, b = P < 0.01 vs. 10 minute CO2 flies.
