Figure 4
From: Computer-assisted quantification of motile and invasive capabilities of cancer cells
Selective induction of cell dissemination with growth factors in the presence of 10% FCS.
(A,B) End-point quantification of % area covered in zone infiltration assay using aZEcs. Means and SDs of three independent experiments with DAOY (A) or UW228 (B) cells at T24h after stimulation with factors as indicated are shown. Concentrations of growth factors/cytokines were as follows: HGF (20 ng/ml), EGF 30 ng/ml), IGF (20 ng/ml), netrin (200 ng/ml), PDGF-B (20 ng/ml), TNF-α (25 ng/ml), bFGF (100 ng/ml), IL-6 (20 ng/ml), NGF (50 ng/ml), TGF-β (20 ng/ml) and PlGF-1 (10 ng/ml) (C,D) Time-lapsed mean and SD quantifications of % area covered from three independent zone infiltration experiments using aZEcs in DAOY (C) or UW228 (D) cells stimulated with factors as indicated. (E) Representative images of 100× magnified microbeads coated with DAOY or UW228 cells after 24 h −/+ stimulation with bFGF, IGF or TGF-beta (DAOY) or EGF, IL-6 or TGF-beta (UW228). LA-EGFP in green, Hoechst staining in blue. Right panels: quantification of means and SDs of cell dissemination/invasion from three independent experiments using aMDIcs. Concentrations of growth factors/cytokines as in 4(A,B). (F) Representative images of 50× magnified spheroids of DAOY or UW228 cells after 24 h −/+ stimulation with bFGF, IGF or TGF-beta (DAOY) or EGF, IL-6 or NGF (UW228); LA-EGFP in green, Hoechst staining in blue. Right panels: Quantification of distance of cell dissemination/invasion using aSDIcs. Concentrations of growth factors/cytokines as in 4(A,B).
