Figure 5
From: Computer-assisted quantification of motile and invasive capabilities of cancer cells
Quantification of growth factor-induced dissemination/invasion of primary MB tumour cells.
(A) Representative images of 50× magnified spheroids of primary MB tumour cells after 24 h −/+ stimulation with bFGF, PIGF and/or PDGF-beta. Inverted greyscale images of Hoechst-stained nuclei are shown. (B) Quantification of distance from centres of spheroids in two experimental replicas using aSDIcs. Concentrations of growth factors/cytokines as in 4(A,B). Box plots with whiskers min to max are shown. Representative images of 50× magnified spheroids of Med PDX 1712 (C) and Med PDX 411 (D) and the quantification of average cell disseminations using aSDIcs. Concentrations of growth factors/cytokines as in 4A,B. Mean and S.D. of three experiments are shown. (E) Dot plot compares average dissemination of Med PDX 1712 (blue) and Med PDX 411 (red) relative to unstimulated controls.
