Figure 1

TCV genome, variants created in this study and their infectivity in Arabidopsis plants.
(A) Schematic representation of TCV genome organization, with the newly introduced KpnI site shown immediately downstream of the CP coding region. The variants A–I, each containing a 21 nt insert at the KpnI site, were depicted beneath the genome. (B) The accumulation levels of variant A–I in the inoculated and systemically infected leaves (ILs and SLs) of Col-0 plants as determined by Northern blot hybridization (NB). The probe used was a 21-nt antisense oligo complementary to the CP coding region (sequence available upon request). EB, ethidium bromide-stained gels serving as loading controls.