Figure 3 | Scientific Reports

Figure 3

From: Random Plant Viral Variants Attain Temporal Advantages During Systemic Infections and in Turn Resist other Variants of the Same Virus

Figure 3

Repression of TCV variants by their pre-inoculated counterparts.

(A,B) A, F, G, H and I are TCV variants differing at a 21 nt region (Fig. 1A). Either variant A or an FGHI variant mix was used to inoculate the proximal half of an IL, followed by a secondary inoculation 48 hours later with the reciprocal variant sets on the distal half of the same IL. Total RNA samples were then collected from ILs (A) and SLs (B) of five independent plants inoculated with A/FGHI (lane 9–13) and FGHI/A (lanes 14–18) and two independent plants of each of the control groups. These samples were then separated on a denaturing agarose gel, transferred to Nylon membranes and subjected to hybridizations with 32P-labeled oligo probes specific for each variant. (C) Sequential inoculations were repeated with variants A and I, with I delayed for o, 6, 12, 24 and 48 hours, respectively.

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