Figure 4 | Scientific Reports

Figure 4

From: Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation

Figure 4

Relationship between the ERK MAPK signaling pathway and HSP60-induced cell migration.

A7r5 cells were treated with 10 ng/ml HSP60 for 0–4 h (a). Cells were treated for 10 min with HSP60 at the indicated concentrations (b). Cells were pretreated with U0126 at the indicated concentrations for 30 min and then stimulated with 20 ng/ml HSP60. The cell lysates were analyzed by western blotting using an antibody against p-ERK. Equal protein loading was confirmed by detection using an ERK antibody (c). The numbers of migrated cells after exposure to HSP60 and the inhibitor were calculated (d). The results are expressed as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 compared with the control. In (a-c), the full length blots were presented in Supplementary Figure S4 (a-c).

Back to article page