Figure 5 | Scientific Reports

Figure 5

From: Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation

Figure 5

Relationship between TLR4 and HSP60-induced cell migration.

A7r5 cells were treated with HSP60 at the indicated concentrations and the expression of TLR4 mRNA was determined by real time RT-PCR (a). Cells were transfected with TLR4 siRNA and the expression of TLR4 mRNA and protein was determined by RT-PCR (b) and western blotting (c). Cells were transfected with TLR4 siRNA and the expression of p-ERK was analyzed by western blotting. Equal protein was confirmed by blotting against an ERK antibody (d). The numbers of migrated cells after treatment with HSP60 and after transfection with TLR4 siRNA were calculated (e). The results are expressed as the means ± SD (n = 3). *p < 0.05 compared with the control. In (b-d), the full length blots were presented in Supplementary Figure S5 (b-d).

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