Figure 3
β-BA increased NO/cGMP levels in vascular endothelium of carotid aortas and HUVECs.
After oral administration with β-BA (100 mg/kg/d or 200 mg/kg/d) for 7 times, NO (a) and cGMP (b) production in carotid arotas of rats were examined by Griess reaction and enzyme-linked immunosorbent assay. All data represent the results (Mean ± SD, n = 8). (#P < 0.05, ##P < 0.01 versus the control group. *P < 0.05, **P < 0.01 versus the model group). (c,d) HEUVCs were pretreated with β-BA for 24 h before being subjected to 6 h OGD then incubated with β-BA for an additional 24 h. NO production was measured by DAF-FM DA. The amount of NO was evaluated by measuring the fluorescence intensity excited at 495 nm and emitted at 515 nm. Representative images were taken by the confocal microscope (bar: 20 μm). All data represent the results (Mean ± SD) of triplicate independent experiments. ##p < 0.01 versus the control group, #p < 0.05 versus the control group, **p < 0.01 versus OGD group, *p < 0.05 versus OGD group).
