Figure 4
Effects of β-BA on the phosphorylation of eNOS at Ser1177 in carotid aortas and HUVECs.
(a) After oral administration with β-BA (100 mg/kg/d or 200 mg/kg/d) for 7 times. Representative immunohistochemistry staining for p-eNOS (Ser1177) was shown on carotid arotas (bar: 100 μm); (b) HEUVCs were pretreated with β-BA for 24 h before being subjected to 6 h OGD then incubated with β-BA for an additional 24 h. Cells were immunostained with anti-phospho eNOS (Ser1177) antibodies, representative immunofluorescent staining for p-eNOS (Ser1177) was shown (bar 20 μm). DAPI: nuclear staining and merged images were also exhibited. Comparison of fluorescence intensity changes in HUVECs are presented by the histogram. (c) Western blot analysis showed the expression of p-eNOS (Ser1177) in HUVECs and the blots were operated under the same experimental conditions. The data represent the results (Mean ± SD) of triplicate independent experiments.
