Figure 1
From: In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications
Characterization of GNPs.
(a) Schematic diagram of the GNP synthesis process: GNPs were conjugated to the linker PEG7 (95%, Sigma-Aldrich, Israel Ltd) followed by covalent conjugation to glucose (GLU) (D-(+)-Glucosamine hydrochloride, Sigma-Aldrich, Israel Ltd.). (b) TEM image of 20 nm GNPs (scale bar 20 nm). (c) Optical properties of GNPs: UV-vis spectroscopy of bare GNPs, PEG7 coated GNPs and Glucose-PEG7 coated GNPs. (d) Mean hydrodynamic diameter obtained by DLS, at room temperature and at a scattering angle of 90°, at the various stages of GNP coatings. DLS measurements were carried out in triplicate. The significant differences obtained (both by DLS and UV-Vis spectroscopy) following each chemical step, demonstrates the efficiency of the chemical coating.
