Figure 8
From: In vivo sub-femtoliter resolution photoacoustic microscopy with higher frame rates
Experimental setup of 2PAM based on laser scanning.
Dispersion pre-compensation was set within the laser to prevent the chirping effect caused by the AOMs in the loss modulation system. The red arrows in the figure represent laser light paths, while the black arrows indicate electrical signal connections. M, mirror; TS, telescope; BS, non-polarizing beam-splitter; AOM, acousto-optic modulator; PD, photo detector; ND, neutral density wheel; O, objective lens; WC, water cavity; T, ultrasonic transducer; PreA1 and PreA2, amplifiers (PR5678 and PR5660B, Olympus) for pre-amplification; F1 and F2, electronic low-pass filters (BLP-15+ and BLP-1.9+, Minicircuit); Ref., reference signal in electrical form; Sig., photoacoustic signals in electrical form; PP, dispersion pre-compensation prism pair; TLI, transmitted light illuminator for bright field observation.
