Figure 2
From: MiR-129-3p promotes docetaxel resistance of breast cancer cells via CP110 inhibition
CP110 is a miR-129-3p target.
(A) The miR-129-3p binding sites in the CP110 3′ UTR are shown, as predicted by bioinformatics analysis. (B) miR-129-3p transfection inhibited firefly luciferase activity of pMIR-REPORT-3′-UTR-CP110 (wild-type, wt) and such inhibition was absent when the miR-129-3p-binding site was mutated (mutant, mu). The effect of miR-129-3p on CP110 expression was normalized and compared to the negative miRNA transfection. (C) Quantitative real-time PCR and western blot analysis of CP110 mRNA and protein expression in parental MDA-MB-231 cells compared to docetaxel-resistant MDA-MB-231/Doc cells. (D) miR-129 overexpression inhibited CP110 protein expression and down-regulation of miR-129-3p facilitated CP110 protein expression. (E) Quantitative real-time PCR analysis of CP110 in MDA-MB-231 and MCF-7 cells stably transfected with miR-129. (F,G) Quantitative real-time PCR analysis of CP110 in MDA-MB-231/Doc and MCF-7 cells transfected with miR-129-3p inhibitor. Each column shows the mean of three independent experiments. Each bar represents the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test.
