Table 2 Name, accession number, primer sequence, probe number, amplicon size, efficiency and R² of 15 candidate HKGs.

HKG Name	Accession Number	Primer Sequence (5′-3′)	UPL Probe Number	Amplicon Size (bp)	Threshold	Efficiency (%) ± SD	R²
ACTB	AB603937.1	F-AGGACCTCTATGCCAACACG	157	75	0.020	97.97 ± 0.668	1.000
		R-CCTTCTGCATCCTGTCAGC
B2M	DQ404542.1	F-GGTGGAGCAATCAGACCTGT	93	78	0.035	95.19 ± 0.056	0.998
		R-GCGTTGGGAGTGAACTCAG
GAPDH	DQ404538.1	F-CACCTCAAGATCGTCAGCAA	119	81	0.020	97.73 ± 0.186	0.999
		R-GCCGAAGTGGTCATGGAT
HPRT1	DQ533610.1	F-GTGGCCCTCTGTGTGCTC	120	81	0.012	96.95 ± 1.441	0.996
		R-ACTATTTCTGTTCAGTGCTTTGATGT
LDHA	AB477023.1	F-TCCACCATGATTAAGGGTTTG	123	97	0.020	97.70 ± 1.782	0.999
		R-CTTTCACAACATCTGAGATTCCA
LDHB	AB477024.1	F-TCGGGGGTTAACCAGTGTT	161	78	0.005	99.94 ± 1.757	0.993
		R-AGGGTGTCTGCACTTTTCTTG
PGK1	DQ533611.1	F-CACTGTGGCCTCTGGCATA	108	84	0.015	97.41 ± 0.824	0.999
		R-GCAACAGCCTCAGCATACTTC
RPL4	DQ404536.1	F-CAGCGCTGGTCATGTCTAAA	119	108	0.035	97.24 ± 0.831	1.000
		R-GCAAAACAGCCTCCTTGGT
RPL8	GQ141092.1	F-CCATGAATCCTGTGGAGCAT	131	65	0.020	102.29 ± 2.102	1.000
		R-GGTAGAGGGTTTGCCGATG
RPL18	DQ403041.1	F-GCAAGATCCTCACCTTCGAC	93	104	0.020	98.04 ± 1.608	0.999
		R-GAAATGCCTGTACACCTCTCG
RPS9	EU638307.1	F-CTGACGCTGGATGAGAAAGAC	155	77	0.020	99.43 ± 0.918	1.000
		R-ACCCCGATACGGACGAGT
RPS18	DQ404537	F-GTACGAGGCCAGCACACC	114	90	0.020	98.98 ± 0.493	0.999
		R-TAACAGACAACGCCCACAAA
SDHA	DQ404540.1	F-CGTATCCCGCTCCATGAC	144	73	0.012	101.70 ± 3.729	0.994
		R-CAGGTACACGTGATCCTTCTCA
TFRC	DQ404541.1	F- TTTAAACCCAGCAGGAGCAT	140	69	0.020	95.36 ± 0.172	0.999
		R- AGTGGCACCAATAGCTCCAA
YWHAZ	DQ404539	F-TCTCTTGCAAAAACGGCATT	135	76	0.003	99.92 ± 2.681	0.995
		R-TGCTGTCTTTGTATGACTCTTCACT

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