Table 3 Results of stability among 15 candidate genes computed by 4 algorithms using 75 bottlenose dolphin blood samples.
HKGs | Comprehensive Ranking | Delta CT | BestKeeper | NormFinder | geNorm | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Geomean of Ranking Value | Rank | Average of SD | Rank | SD | Rank | Stability value | Rank | M value | Rank | |
RPL4 | 2.21 | 1 | 0.621 | 3 | 0.540 | 1 | 0.390 | 2 | 0.300 | 4 |
HPRT1 | 2.89 | 2 | 0.616 | 2 | 0.608 | 5 | 0.357 | 1 | 0.515 | 7 |
PGK1 | 3.83 | 3 | 0.612 | 1 | 0.664 | 9 | 0.408 | 3 | 0.572 | 8 |
RPL18 | 5.07 | 4 | 0.682 | 10 | 0.626 | 6 | 0.536 | 11 | 0.178 | 1 |
RPS18 | 5.18 | 5 | 0.676 | 8 | 0.667 | 10 | 0.520 | 9 | 0.178 | 1 |
RPS9 | 5.21 | 6 | 0.675 | 7 | 0.579 | 3 | 0.492 | 7 | 0.334 | 5 |
B2M | 5.42 | 7 | 0.678 | 9 | 0.604 | 4 | 0.452 | 4 | 0.440 | 6 |
ACTB | 6.82 | 8 | 0.636 | 4 | 0.738 | 12 | 0.460 | 5 | 0.606 | 9 |
RPL8 | 7.50 | 9 | 0.700 | 11 | 0.635 | 8 | 0.558 | 12 | 0.237 | 3 |
GAPDH | 7.58 | 10 | 0.668 | 5 | 0.717 | 11 | 0.490 | 6 | 0.621 | 10 |
TFRC | 8.61 | 11 | 0.766 | 14 | 0.558 | 2 | 0.597 | 14 | 0.665 | 14 |
LDHA | 9.10 | 12 | 0.674 | 6 | 0.739 | 13 | 0.520 | 8 | 0.630 | 11 |
LDHB | 10.43 | 13 | 0.722 | 13 | 0.628 | 7 | 0.522 | 10 | 0.649 | 13 |
YWHAZ | 12.94 | 14 | 0.715 | 12 | 0.783 | 15 | 0.571 | 13 | 0.638 | 12 |
SDHA | 14.74 | 15 | 0.796 | 15 | 0.748 | 14 | 0.641 | 15 | 0.682 | 15 |