Table 4 Results of stability among 15 candidate genes computed by 4 algorithms using 55 bottlenose dolphin blood samples.
HKGs | Comprehensive Ranking | Delta CT | BestKeeper | NormFinder | geNorm | |||||
---|---|---|---|---|---|---|---|---|---|---|
Geomean of Ranking Value | Rank | Average of SD | Rank | SD | Rank | Stability value | Rank | M value | Rank | |
PGK1 | 2.21 | 1 | 0.612 | 1 | 0.657 | 8 | 0.398 | 3 | 0.242 | 1 |
HPRT1 | 3.08 | 2 | 0.633 | 3 | 0.624 | 5 | 0.376 | 1 | 0.386 | 6 |
RPL4 | 3.13 | 3 | 0.619 | 2 | 0.578 | 3 | 0.382 | 2 | 0.539 | 8 |
RPS9 | 3.87 | 4 | 0.665 | 5 | 0.565 | 1 | 0.469 | 5 | 0.585 | 9 |
ACTB | 4.20 | 5 | 0.649 | 4 | 0.740 | 13 | 0.477 | 6 | 0.242 | 1 |
B2M | 5.09 | 6 | 0.671 | 6 | 0.593 | 4 | 0.435 | 4 | 0.474 | 7 |
LDHA | 6.34 | 7 | 0.685 | 7 | 0.732 | 11 | 0.529 | 7 | 0.247 | 3 |
GAPDH | 7.87 | 8 | 0.698 | 10 | 0.736 | 12 | 0.535 | 8 | 0.283 | 4 |
TFRC | 8.61 | 9 | 0.755 | 14 | 0.577 | 2 | 0.570 | 14 | 0.669 | 14 |
RPS18 | 9.43 | 10 | 0.691 | 8 | 0.685 | 10 | 0.540 | 9 | 0.635 | 11 |
RPL8 | 9.49 | 11 | 0.694 | 9 | 0.665 | 9 | 0.544 | 10 | 0.619 | 10 |
YWHAZ | 9.80 | 12 | 0.706 | 12 | 0.743 | 14 | 0.544 | 11 | 0.312 | 5 |
RPL18 | 10.26 | 13 | 0.698 | 11 | 0.644 | 7 | 0.559 | 12 | 0.642 | 12 |
LDHB | 10.72 | 14 | 0.751 | 13 | 0.638 | 6 | 0.561 | 13 | 0.656 | 13 |
SDHA | 15.00 | 15 | 0.824 | 15 | 0.747 | 15 | 0.671 | 15 | 0.690 | 15 |